Wright Stain is a routine staining method in hematology and cytology used to examine the morphology of blood cells in peripheral blood smears or bone marrow samples. This staining technique is a combination of eosin (an acidic dye) and methylene blue (a basic dye) that selectively colors cellular components based on their acid-base properties. The nuclei of white blood cells (such as lymphocytes and granulocytes) appear blue-purple, the cytoplasm stains pink or light blue, and specific granules in cells like neutrophils and eosinophils are clearly distinguishable. Wright stain is widely used in the diagnosis of blood disorders such as anemia, leukemia, parasitic infections (like malaria), and cellular abnormalities. Due to its simplicity, speed, and low cost, it is considered one of the essential tools in clinical laboratories. The quality of staining depends on factors such as staining time, buffer solution pH, and the thickness of the blood smear.

